(authors)Fluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model.Metildi, Cristina A Kaushal, Sharmeela Snyder, Cynthia S Hoffman, Robert M Bouvet, MichaelWe inquired if fluorescence-guided surgery (FGS) could improve surgical outcomes in fluorescent orthotopic nude mouse models of human colon cancer. We established fluorescent orthotopic mouse models of human colon cancer expressing a fluorescent protein. Tumors were resected under bright light surgery (BLS) or FGS. Pre- and post-operative images with the OV-100 Small Animal Imaging System (Olympus Corp, Tokyo Japan) were obtained to assess the extent of surgical resection. Tumor diameter was measured every other day.Īll mice with primary tumor that had undergone FGS had complete resection compared with 58% of mice in the BLS group (P = 0.001).
Percentage of tumor growth inhibition, growth delay time and regrowth delay time were evaluated. Results: For A549 xenografts in radiation alone, gefitinib alone and combination therapy groups, the percentage of tumor growth inhibition was 22.7%, 12.4% and 38.2%, respectively (F=25.75, P=0.000). Tumor regrowth delay time in combination therapy and irradiation alone groups was 23.4 and 10.2 days. Sensitizing enhancement ratio of combination group was 1.5 in growth and 1.7 in regrowth. The mice were derided into 4 groups: control, irradiation alone, Gefinitib alone and radiation combined with Genifitib. Radiation schedule was 3 fractions of 5 Gy, once daily. Gefitinib was daily administered by gavage at 100 mg/(kgÂ
EARLY SANDRA ORLOW NUDE FULL TEXT AVAILABLE.Sandra Ann Lauer was born in the German town of Saarbrücken, close to the French border. Her father, Robert Lauer, who owned a wine store in Saarbrücken, is French and her mother, Karin ( née Eltern), who worked in a shoe store, is German. She also had an older brother, Gaston, a paraplegic who died in 1995.
Sandra showed an early interest in music and dancing, starting to learn classical ballet at the age of 5, which she would continue for ten years, and receiving guitar lessons when she was 10 years old. In 1975, at the age of thirteen, Sandra went with her mother to see Young Star Festival, a Saarbrücken talent competition. She was only a member of the audience, but when all participants had finished performing and the jury was discussing the results, Sandra walked onto the stage, persuaded the DJ to put on the German version of a song made famous by Olivia Newton-John and started singing. The impromptu performance gained considerable recognition and led to the release of her first single, which was a children's song about a pet dog, 'Andy mein Freund'. The single, however, performed poorly on charts at that time dominated by disco mania. Sandra won international success in 1985 with a song '(I'll Never Be) Maria Magdalena', which topped the charts in 21 countries worldwide and reached top 10 in further five. Her first album, The Long Play (1985), reached number 12 in her home country of Germany and was a top 10 success in Scandinavia. The follow-up single, 'In the Heat of the Night', continued her international success, reaching number two in Germany and top 10 positions in many European countries. The song also earned Sandra second place at the Tokyo Music Festival in 1986. 'Little Girl' became the third single from the album in 1986, with the music video filmed in Venice, but met with moderate success. Shortly after the release of The Long Play, Sandra moved to London for six months, where she worked with singing instructor Helena Shelen and took drumming lessons to practice her timing. She also joined a language school, where she worked on her English skills, while she spent weekends in Munich recording new songs. Sandra and Michael married in January 1988 and relocated from Munich to the Spanish island Ibiza to work on what would be Sandra's third studio album. Into a Secret Land moved from electro-pop to more sophisticated areas of pop, what was showcased by the first single, 'Heaven Can Wait', a top 20 European hit.